Alexander Leonidovich Khokhlov1,2, Yuriy Aleksandrovich Dzhurko2 , Ilya Igorevich Yaichkov1,2*, Leonid Nikolaevich Shitov1,2, Anastasiya Mikhailovna Shitova2,3, Lidia Alexandrovna Khozova2 , Aleksey Evgenyevich Miroshnikov1
1State Budget-funded Educational Establishment of Higher Professional Education, Yaroslavl State Medical University of the Ministry of Health of the Russian Federation, Yaroslavl, Russia
2Quinta-Analytica Yaroslavl LLC, Yaroslavl, Russia.
3State Educational Establishment of Higher Professional Education, Yaroslavl State University named after P. G. Demidov of the Ministry of Education and Science of the Russian Federation, Yaroslavl, Russia.
Corresponding Author: Ilya Igorevich Yaichkov, 150043, Avtozavodskaya st, 29-97, Yaroslavl, Russian, Tel: 89109777498; E-Mail: [email protected]
Received Date: 10 Feb 2017
Accepted Date: 14 Feb 2017
Published Date: 27 Feb 2017
Copyright © 2017 Yaichkov II
Citation: Khokhlov AL, Dzhurko YA, Yaichkov II, Shitov LN, et al. (2017). The Rapid and Sensitive Hplc-Ms/Ms-Method of Determination of Mebeverine Metabolites in Human Plasma.Mathews J Pharm Sci. 2(1): 010.
The rapid and sensitive method for quantifying mebeverine metabolites in human plasma was developed using HPLC-MS/ MS. Mebeverine acid-D5 and desmethyl mebeverine acid-D5 were used as internal standards. Sample preparation was performed by protein precipitation. The chromatographic separation was achieved on Phenomenex Luna C8 Mercury (20 x 4.0 mm, 5 μm) and Phenomenex Luna 5u C8 (150 x 4.6 mm, 5 μm) under gradient conditions of mobile phase. The calibration range of mebeverine acid and desmethyl mebeverine acid was 10 - 2000 ng/ml. The method was applied to conduct a pharmacokinetic study of 200 mg prolonged-release capsules Duspatalin on 24 healthy participants.
HPLC-MS/MS; Mebeverine Acid; Desmethyl Mebeverine Acid; Pharmacokinetic.