Mathews Journal of Immunology & Allergy


Current Issue Volume 7, Issue 2 - 2023

Presence Of Plasmocytes in Non-Immunized A. Rubens Sea Stars

Michel Leclerc*

Immunology of Invertebrates, Orléans University, France

*Corresponding author: Michel Leclerc, Immunology of Invertebrates, Orléans University, France, Tel: 0238410209, Email: [email protected].

Received Date: November 27, 2022

Published Date: December 08, 2023

Citation: Leclerc M. (2023). Presence Of Plasmocytes in Non-Immunized A. Rubens Sea Stars. Mathews J Immunol Allergy. 7(2):24.

Copyrights: Leclerc M. © (2023).


In this brief report, we recall the existence of sea star T and B lymphocytes in the sea star Asterias rubens. Otherwise the figure 1 shows sea star Plasmocytes, after centrifugation in a micro hematocrit centrifuge at 100g, in animals which have not been immunized. Their presence calls an explanation: Either they exist in a spontaneous way or, may be, they are induced by external immune causes.

Keywords: Plasmocytes, Sea Stars, Animals, Axial Organ, Lymphocytes.


We have described in the past [1] plasmolymphocytic cells in the Axial Organ (AO) of starfishes or sea stars: Asterias rubens, after immunizations to HRP (Horse-radish peroxydase). More recently, we discover what we call sea star plasmocytes, in non-immunized A.rubens ( Figure 1), at the level of the Axial organ, which has been considered by us, as a primitive lymphoïd organ. It merits to be said because it s the only lymphoïd organ which has been discovered in Invertebrates [2].


The AO cells (from the whole AO cell population), were obtained after centrifugation at 100 g, in a micro hematocrit centrifuge and colored to Giemsa.


The Figure 1 shows plasmocytes of A.rubens with a diameter of 7-8 µ: they have a cytoplasm more important than in lymphocytes (either sea star T lymphocytes or B sea star lymphocytes [3]. We recall sea star lymphocytes can be separated into T and B by a nylon-wool column, according the well-known method of Julius et al. [4].

The plasmocyte nucleus seem reoriented in the Figure 1: it is due to the centrifugation. Some of them are reniform (3 nuclei out of 4 observed). At last some vesicles appear in the cytoplasm.

Figure 1. Sea star Asterias rubens plasmocytes after centrifugation and coloration at Giemsa.


The existence of sea star A.rubens plasmocytes in non-immunized A.rubens can be explained by:

The fact:

  1. They exist in a spontaneous way.
  2. Some external attacks like virus, microbes which surround the biotope can provoke their emergence.

We presume that sea star plasmocytes are issued from the lineage of B sea star cell subpopulation. It is correlated, in conclusion, to the existence of the IPA (Invertebrate Primitive Antibody) and also Nanobodies in Invertebrates [5]. It seems necessary to repeat again these data to the Scientific Communauty.


  1. Leclerc M. Thèse de Doctorat ès Sciences. France: Orleans University; 1974.
  2. Leclerc M,  Brillouet C,  Luquet G. (1980). The starfish axial organ: An ancestral lymphoid organ. Dev Comp Immunol. 4(4):605-615.
  3. Leclerc M, Arneodo V, Legac E, et al. (1993). Identification of T-like and B-like lymphocyte subsets in sea star Asterias rubens by monoclonal antibodies to human leucocytes. Thymus. 21:133-139.
  4. Julius MH, Simpson E, Herzenberg LA. (1973). A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 3(10):645-649.
  5. Leclerc M. (2022). The Nanobodies and its Relations to IPA (Invertebrate Primitive Antibodies). Existence of At Least 2 Classes of IPA in Sea Star Immune System. Mathews J Immunol Allergy. 6(1):14.

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