Human Immunodeficiency Virus Type 1 and Type 2 Co-infection Rate in Kinshasa Patients
Erick Ntambwe Kamangu1
1Service of Molecular Biology, Department of Basic Sciences, Faculty of Medicine, University of Kinshasa(UNIKIN), Kinshasa- Democratic Republic of Congo.
Corresponding Author:Erick Ntambwe Kamangu, Service of Molecular Biology, Department of Basic Sciences, Faculty of Medicine,
University of Kinshasa (UNIKIN), Kinshasa-Democratic Republic of Congo, Tel: +243 (0) 81 54 01;
- Received Date: 26 Jul 2016 Accepted Date: 18 Aug 2016 Published Date: 20 Aug 2016
- Copyright © 2016 Kamangu EN
Citation: Kamangu EN. (2016). Human Immunodeficiency Virus Type 1 and Type 2 Co-infection Rate in Kinshasa Patients. M J HIV. 1(1): 009.
Although the two types of HIV essentially cause AIDS; there are major differences between them in terms of phylogeny, pathology, virulence, evolution, transmission, infectivity and epidemiology.Objective:
The objective of this study was to determine the rate of HIV-1 and HIV-2 co-infection in people infected with HIV in Kinshasa.Methodology:
A cross-sectional study performed on 115 randomly selected plasma samples in the archived plasma samples of patients infected with HIV-1. 140µl were used for the extraction of RNA from plasma using the kit QIAamp® RNA Mini Kit in the Molecular Biology Laboratory. A final eluate of 60µl was collected. Different qualitative real-time PCR were performed on all 115 samples to determine HIV-1 and HIV-2 infection.Results:
113 samples were positive for HIV-1. Four samples of these positive samples also tested positive for HIV-2 giving a rate of HIV-1 and HIV-2 coinfection of 3.54%.Conclusion:
The rate of coinfection HIV-1 and HIV-2 is 3.54% for Kinshasa. This coinfection questions the notions of HIV-2 for the city and even for the Democratic Republic of Congo.
KEYWORDSCoinfection; HIV-1; HIV-2; Kinshasa.
The Human Immunodeficiency Virus (HIV) is classified into
two types (HIV-1 and HIV-2) . Although these two types of
HIV (HIV-1 and HIV-2) cause essentially the Acquired Immune
Deficiency Syndrome (AIDS); there are major differences between
them. Their phylogenies, pathology, virulence, developments,
modes of transmission, infectivity and epidemiology
differ . Compared to the HIV-1, the HIV-2 has a slower
progression to AIDS stage and is hardly transmitted . Coding
regions of the two types of HIV vary . The Type 1 is the most
common HIV worldwide; it is responsible for over 80% of the
global epidemic [1, 3]. The Subtype C of Group M is the most
prevalent. It is responsible for over a quarter of the global in
fection [3, 4]. It is followed by subtype B of the same group; it
is found in the industrialized countries of Western Europe and
North America [3, 4], while infection with HIV-2 is less spread
and is confined mainly in West Africa [2, 5]. Additionally, the
HIV-2 AIDS progression is much slower and the virus is hardly
transmitted more easily because of its low infectivity and its
slow progress; especially as the Viral Loads (VL) in individuals
infected with HIV-2 are significantly lower [2, 6, 7].
Currently, no HIV-2 has been documented in Kinshasa or even in the Democratic Republic of Congo (DRC). Hence, the objective of this study was to determine the rate of HIV-1 and HIV-2 co-infection in Kinshasa population.
This study was a cross-sectional study on 115 randomly selected
plasma samples from the archived plasma samples of
newly infected individuals with HIV-1 without Antiretroviral
treatment collected between 2013 and 2014 [8, 9]. 500µl of
plasma samples were stored at -20°C.
RNA extraction and amplification 140µl were used to extract RNA from plasma using the kit QIAamp® RNA Mini Kit (QIAGEN, Hilden, Germany) in the Laboratory of Molecular Biology to collect a final eluate of 60µl . For confirmation of the infection by HIV-1, a qualitative real-time PCR, based on the previously referred test, was performed on all 115 samples . Each sample (10µl) was analyzed in duplicate.
A qualitative real-time PCR was conducted to determine the HIV-2 infection according to the protocols described previously [12, 13]. Each sample (10µl) was also analyzed in duplicate.
In case of discrepancy between the PCR results, a 3rd Qualitative Real Time PCR (a triplet) was performed.
All analysis were performed at Laboratory of Molecular Biology of the Department of Basic Sciences of the Faculty of Medicine of the University of Kinshasa.
113 samples out of 115 tested were positive for HIV-1, giving an amplification rate of 98.3%. Four samples of the 113 were positive for HIV-2 giving a rate of HIV-1 and HIV-2 coinfection of 4/113 samples or 3.54% for a population newly infected and naive of antiretroviral treatment.
113 samples of the 115 analyzed (98.3%) were positive for
HIV-1 using qualitative real-time PCR based on a previously
described test. Two samples which were previously positive
, did not amplified even though the PCR tests used were
almost same. This difference can be caused by a conservation
problem or even thawing and freezing. Indeed, after freezing
and thawing, a sample loose close to 1.00 log10 . This
means that samples which previously had low VL, i.e., less
than this threshold, should not be amplified after thawing
and freezing. This justifies the two samples that were not amplified
in the collection. These archived plasma samples were
previously used in other studies [8, 9, 11].
Four samples out of 113 tested positive for HIV Type 2 by qualitative real-time PCR giving HIV-1 and HIV-2 co-infection rate of 4/113 or 3.54% for samples from a population of newly infected patient’s naďve of treatment for HIV in Kinshasa. No study to date has shown that coinfection in Kinshasa; these results challenge the knowledge of the geographical distribution of HIV-2.Our study uncovers the presentation of significant AH in HIV infected individuals who were selected randomly. Further research should be performed to look into histopathological examination and study of the trends of CD4 counts associated with AH.
This study was conducted to determine the HIV coinfection rate from patients newly infected, using samples from the archived plasma samples of the Laboratory of Molecular Biology. The rate of coinfection HIV-1 and HIV-2 is of 3.54% for Kinshasa. This coinfection questions the notions of HIV Type 2 for the city of Kinshasa and even the Democratic Republic of Congo.