Mathews Journal of Dentistry

2474-6843

Previous Issues Volume 3, Issue 1 - 2018

Research Article PDF  

Evaluation of p53, Ki-67 and c-erb-B2 Expression in Normal Oral Epithelium, Dysplastic Epithelium, and Oral Squamous Cell Carcinoma

Banu Ozveri Koyuncu1,Cemal Akay2, Banu Yaman3, Ali Veral4,Sevtap Gunbay5,Tayfun Gunbay5

1Associate Professor, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Ege.

2Professor, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Ege University, Bornova, Izmir, Turkey.

3Associate Professor, Department of Pathology, Faculty of Medicine, Ege University, Bornova, Izmir, Turkey.

4Professor, Department of Pathology, Faculty of Medicine, Ege University, Bornova, Izmir,Turkey.

5Professor, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Ege University, Bornova, Izmir, Turkey.

Corresponding Author: Banu Ozveri Koyuncu, Associate Professor, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Ege, Tel: 90-232-3881108; E-Mail: banuozverikoyuncu@yahoo.com

Received Date: 02 Oct 2018  
Accepted Date: 09 Oct 2018   
Published Date: 11 Oct 2018
Copyright © 2018 Koyuncu BO

Citation: Koyuncu BO, Akay C, Yaman B, Veral A, et al. (2018). Evaluation of p53, Ki-67 and c-erb-B2 expression in normal oral epithelium, dysplastic epithelium, and oral squamous cell carcinoma. Mathews J Dentistry. 3(1): 019.

 

ABSTRACT

The expression of p53, Ki-67, and c-erb-B2 in normal oral epithelium, dysplastic epithelium, and oral squamous cell carcinoma (OSCC) was investigated with immunohistochemical method and was determined the correlation between them. The study was performed on tissue sections of dysplasia (n= 25), OSCC (n=22). Twenty normal samples of oral epithelium were used as control. Sections were stained with immunohistochemically with antibodies to p53, Ki-67, and c-erb-B2 proteins. The percentage of positive cells was assessed in oral dysplastic, OSSC and normal epithelium. Statistical analysis was done using Kruskal-Wallis, Mann Whitney U, and the chi-square tests. The percentage of p53 and Ki-67 positive cells was evaluated and statistically significant differences were found between normal oral mucosa and dysplasia, and between dysplasia and invasive oral squamous cell carcinoma (p<0.05). C-erb-B2 positive cells increased from normal oral epithelium through dysplasia to OSCC, but it was not statistically important (p>0.05). A significant correlation between p53 and Ki-67 positive cells was observed (p<0.001) but no significant correlation was found between c-erb-B2, p53 and Ki-67 in OSCC (p>0.05). These findings suggested that the expression of p53 and increase of Ki-67 index may play roles in carcinogenesis.

 

KEYWORDS

Oral Squamous Cell Carcinoma; Dysplasia; Ki-67; p53; c-erb-B2; Immunohistochemical; Carcinogenesis.


© 2015 Mathews Open Access Journals. All Rights Reserved.

Creative Commons License
Open Access by Mathews Open Access Journals is licensed under a
Creative Commons Attribution 4.0 International License.
Based On a Work at Mathewsopenaccess.com